Host-vector system for antibiotic-free cole1 plasmid...

Details Host-vector system for antibiotic-free cole1 plasmid... Host-vector system for antibiotic-free cole1 plasmid...

C - Chemistry, Metallurgy

12

N

C12N 15/70 (2006.01)

Patent

CA 2580009

A host-vector system that uses the RNA-based copy number control mechanism of ColE1-type plasmids for regulating the expression of a marker gene allows for antibiotic-free selection of plasmids and is useful for production of plasmid DNA and recombinant proteins.

L'invention porte sur un système de vecteur hôte qui utilise le mécanisme de régulation du nombre de copies à base d'ARN des plasmides de type Co1E1 pour réguler l'expression d'un gène marqueur, ce qui permet une sélection sans antibiotiques des plasmides, ce système étant utile dans la production d'ADN plasmidique et de protéines de recombinaison.

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