Hosts and fermentation processes for cellulase production

Details Hosts and fermentation processes for cellulase production Hosts and fermentation processes for cellulase production

C - Chemistry, Metallurgy

12

N

C12N 9/42 (2006.01) C07K 14/37 (2006.01) C12N 1/15 (2006.01) C12N 15/09 (2006.01) C12N 15/80 (2006.01)

Patent

CA 2742144

A fermentation process for the production of cellulase mix-tures is provided. The process comprises providing a genetically modified host filamentous fungus that overexpresses a Xyrl transcription factor and/ or that is partially or completely deficient in expressing one or more hemi-cellulase enzyme. The host filamentous fungus is cultured in a medium comprising a carbon source. The carbon source contains from about 60 wt % to about 100 wt% hemicellulose-derived carbohydrate and less than 5% of a cellulase-inducing carbohydrate or contains from about 25 wt% to about 100% wt % hemicellulose-derived sugar alcohol in combination with from about 0 wt% to about 75 wt% glucose, glycerol or a combina-tion thereof.

L'invention porte sur un procédé de fermentation pour la production de mélanges de cellulases. Le procédé comprend la fourniture d'un champignon filamenteux hôte génétiquement modifié qui surexprime un facteur de transcription Xyrl et/ou qui est partiellement ou totalement déficient en ce qui concerne l'expression d'une ou plusieurs enzymes hémicellulases. Le champignon filamenteux hôte est mis en culture dans un milieu comprenant une source de carbone. La source de carbone contient d'environ 60 % en poids à environ 100 % en poids de glucide issu d'hémicellulose et moins de 5 % en poids d'un glucide induisant une cellulase ou contient d'environ 25 % en poids à environ 100 % en poids d'alcool de sucre issu d'hémicellulose en association avec d'environ 0 % en poids à environ 75 % en poids de glucose, de glycérol ou d'une association de ceux-ci.

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