How to affect the chlorophyll biosynthesis in plants

C - Chemistry – Metallurgy – 12 – N

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C12N 15/82 (2006.01) A01H 5/00 (2006.01) C07K 16/14 (2006.01) C12N 9/10 (2006.01) C12N 9/90 (2006.01) C12N 15/54 (2006.01) C12Q 1/68 (2006.01) G01N 33/50 (2006.01)

Patent

CA 2298100

A gene encoding a hybrid protein consisting of ALA-S yeast and a N-terminal transit sequence for the small carboxydismutase sub-unit is introduced into the genim of tobacco (nicotania tabacum) and a chlorophyll-poor tobacco transformant which expresses glutamate-1-semialdehyde-transminase (GSA-AT) antisense RNA. The expression of ALA-S in the GSA-AT transgenic antisense strain has produced green co-transformants, the chlorophyll content of which is similar to that of the wild type, while the wild-type transformants have shown no phenotypical changes. The capacity of plants to synthetize ALA and chlorophyll is increased, thereby demonstrating the contribution of ALA-S to the ALA supply required for the chlorophyll synthesis. The transgenic plants produce chlorophyll in the presence of gabaculine, a GSA-AT inhibitor. The permanent RNA and protein content of GSA-AT have been reduced, as well has the enzymatic activity of plants expressing ALA-S. The additional synthesis of ALA also explains the expression of GSA-AT in the C5 way.

Un gène codant une protéine hybride composée de la levure ALA-S et d'une séquence de transition N-terminale pour la petite sous-unité de carboxydismutase, est introduit dans le génome du tabac (nicotania tabacum) et d'un transformant de tabac à déficit de chlorophylle, qui exprime de l'ARN antisens à base de glutamate-1-sémialdéhyde-transaminase (GSA-AT). L'expression d'ALA-S dans la lignée antisens transgénique GSA-AT a donné des co-transformants verts dont la teneur en chlorophylle est similaire à celle du type sauvage, tandis que les transformants du type sauvage n'ont pas présenté de modifications phénotypiques. La capacité des plantes à synthétiser l'ALA et la chlorophylle s'en est trouvée accrue. Cela met en évidence la contribution de l'ALA-S à l'alimentation en ALA pour la synthèse de la chlorophylle. Les plantes transgéniques forment de la chlorophylle en présence de gabaculine, un inhibiteur de la GSA-AT. Les teneurs permanentes en ARN et en protéines de la GSA-AT ont été réduites, comme d'ailleurs l'activité enzymatique dans les plantes exprimant de l'ALA-S. La synthèse supplémentaire de l'ALA a aussi permis d'expliquer cette expression de GSA-AT dans la voie C5.

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