Human activated protein c and process for preparing same

Details Human activated protein c and process for preparing same Human activated protein c and process for preparing same

C - Chemistry, Metallurgy

12

N

C12N 9/50 (2006.01) C12N 9/64 (2006.01)

Patent

CA 2175201

The present invention is directed to a human Activated Protein C (APC) preparation with a high specific activity being substantially free from thrombin or an equivalent protease and/or. Protein C not being subjected to activation and a process for preparing the same. The invention is further directed to a human Activated Protein C preparation which shows specific activity of 3500 U/mg or more, the unit of specific activity being defined as an amount of APC which prolongs by two times the activated thromboplastin time (APTT) of normal human plasma, and is substantially free from thrombin or an equivalent protease and/or Protein C not being subjected to activation. Said preparation is prepared by a process for purification of human Activated Protein C which comprises, after activation of Protein C with thrombin or an equivalent protease, contacting a solution containing human Activated Protein C with a cation exchanger to allow for adsorption of thrombin and Activated Protein C to the exchanger, and then eluting human Activated Protein C alone.

La présente invention concerne une préparation de protéine C humaine activée, à activité spécifique élevée et substantiellement exempte de thrombine ou d'une protéase équivalente, et/ou de protéine C non activée. Cette préparation a une activité spécifique supérieure à 3500 V/mg au test dans lequel le temps de céphaline activé sert d'indice. Le procédé de production consiste à purifier une protéine C humaine activée par activation de la protéine C au moyen de thrombine, etc., à mettre une solution contenant la protéine C en contact avec un échangeur cationique absorbant la thrombine et la protéine C activée, à séparer par élution seulement la protéine C activée.

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