Human and mammalian dna replication origin consensus sequences

Details Human and mammalian dna replication origin consensus sequences Human and mammalian dna replication origin consensus sequences

C - Chemistry, Metallurgy

12

N

C12N 15/85 (2006.01) C12N 15/11 (2006.01) C12N 15/90 (2006.01)

Patent

CA 2274306

The present invention relates to a human or mammalian DNA replication origin consensus sequence which consists of a sequence selected from the group consisting of CCTMDAWKSGBYTSMAAWYWBCMYTTRSCAAATTCC (SEQ ID NO:1); and AWMTWAAKRAWRWWKKDAVWWGAKRWWKWVWHRASSACMDWKAAKTWKGGWTWARRYWKGRKMWWTWKAWSDATAKWWW KDAKWKMWRKTT (SEQ ID NO:4). A method for the control of initiation of mammalian DNA replication which comprises the steps of: a) inserting a consensus sequence coding for a sequence of the present invention together with a DNA fragment to form a vector capable of expression of the DNA fragment; b) introducing the vector of step a) into mammalian cells in vitro.

L'invention concerne une séquence consensus de l'origine de réplication d'ADN humain ou d'ADN de mammifère, qui est constituée d'une séquence choisie dans le groupe comprenant CCTMDAWKSGBYTSMAAWYWBCMYTTRSCAAATTCC (SEQ ID NO:1) et AWMTWAAKRAWRWWKKDAVWWGAKRWWKWVWHRASSACMDWKAAKTWKGGWTWARRYWKGRKMWWTWKAWSDATAKWWWKDAKWKMWRKTT (SEQ ID NO:4). Elle concerne également une méthode permettant de contrôler l'initiation de la réplication de l'ADN d'un mammifère, selon laquelle: a) on insère une séquence consensus codant pour une séquence de la présente invention en même temps qu'un fragment d'ADN, de façon à former un vecteur capable d'exprimer ledit fragment; et b) on introduit le vecteur de l'étape a) dans des cellules de mammifère in vitro.

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