Human artificial chromosome containing human antibody...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/09 (2006.01) A01K 67/027 (2006.01) C07K 16/00 (2006.01) C07K 16/24 (2006.01) C12N 15/85 (2006.01) C12N 15/90 (2006.01) C12P 21/08 (2006.01) A61K 48/00 (2006.01)

Patent

CA 2446968

A human artificial chromosome which can be transferred to offspring (the next generation) at a high efficiency and method of using the same. Namely, a human artificial chromosome wherein a domain of about 3.5 Mb to about 1 Mb containing an antibody light chain gene originating in the human 22nd chromosome is ligated to a chromosome fragment originating in another human chromosome, which can be transferred to offspring, via a nonhuman animal genital system; a nonhuman animal carrying this artificial human chromosome and its offspring; a method of constructing the nonhuman animal; a method of constructing a human antibody using the nonhuman animal or its offspring; and a human antibody-producing mouse having the above artificial human chromosome.

L'invention porte sur un chromosome humain artificiel pouvant être transféré sur un rejeton (de la prochaine génération) avec une grande efficacité, et sur son procédé d'utilisation. Il s'agit d'un chromosome humain artificiel dans lequel un domaine d'environ 3,5 Mb à environ 1 Mb contenant le gène à chaîne légère de l'anticorps humain .lambda. provenant du 22?ème¿ chromosome humain est lié à un fragment de chromosome provenant d'un autre chromosome humain, et qui peut être transféré sur un rejeton via le système génital d'un animal. L'invention porte également sur un animal porteur de ce chromosome humain artificiel et sur ses rejetons et sur un procédé d'élaboration d'un anticorps humain par l'intermédiaire d'un animal ou de ses rejetons, et sur une souris produisant un anticorps humain présentant le chromosome humain artificiel ci dessus.

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