Human calcium channels alfa1 subunits and related probes,...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/12 (2006.01) C07K 14/705 (2006.01) C12N 5/10 (2006.01) C12Q 1/68 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2281984

Partial sequences for a novel mammalian (human and rat sequences identified) calcium channel subunit which we have labeled as the .alpha.II subunit, and an additional novel human calcium channel which we have labeled as the .alpha.IH subunit are provided. Knowledge of the sequence of these two calcium channels permits the localization and recovery of the complete sequence from human cells, and the development of cell lines which express the novel calcium channels of the invention. These cells may be used for identifying compounds capable of acting as agonists or antagonists to the calcium channels.

Cette invention concerne des séquences partielles d'une nouvelle sous-unité de canal à calcium de mammifère (les séquences identifiées proviennent d'homme et de rat) qui ont été désignées sous le nom de sous-unité .alpha.¿II? et un autre nouveau canal à calcium humain appelé sous-unité .alpha.¿IH?. La connaissance de la séquence de ces deux canaux à calcium permet de localiser et de récupérer la séquence complète dans des cellules humaines et de développer des lignées cellulaires qui expriment les nouveaux canaux à calcium de cette invention. On peut utiliser ces cellules pour identifier des composés capables d'agir en tant que'agonistes ou qu'antagonistes de ces canaux à calcium.

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