Human cell line and triomas, antibodies, and transformants...

Details Human cell line and triomas, antibodies, and transformants... Human cell line and triomas, antibodies, and transformants...

C - Chemistry, Metallurgy

12

N

195/1.102

C12N 5/22 (2006.01) C12N 5/16 (2006.01) C12P 21/08 (2006.01)

Patent

CA 1315715

Abstract of the Disclosure A stable, continuous human cell line or progeny thereof is produced that is resistant to 6-thioguanine and ouabain, secretes less than 40 ng/ml of endogenous IgM antibodies, and grows with a doubling time of about 18 hours. The cell line, which preferably is adapted to serum-free medium, may be used as a fusion partner with an antibody- producing cell line so as to generate antibodies. In addition, it may be electroporated with a vector containing a gene of interest to produce a transformed cell line which generates a protein encoded by the gene, such as an IgG or IgM antibody.

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