Human cord blood derived unrestricted somatic stem cells (ussc)

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 5/073 (2010.01) C12N 5/0735 (2010.01) A61K 35/14 (2006.01) A61K 35/12 (2006.01)

Patent

CA 2426987

A composition in human cord and placental blood which comprises unrestricted somatic stem cells is described here which can be amplified in vitro to large quantities sufficient for medical applications as regenerative medicines. Initiation and maintenance as well as ex vivo expansion protocols of such stem cells from cord blood is described.Furthermore, it is shown that from these cells employing varying differentiation induction protocols distinct lineage progenitors for hematopoiesis and endothel, as well as mesenchymal progenitors for muscle bone, cartilage and fat as well as neural progenitors can be cultured and expanded for use in regenerative medicine.

L'invention concerne une composition de sang placentaire et cordonal humain comprenant des cellules souches somatiques libres, pouvant être amplifiée in vitro en grandes quantités, suffisantes pour des applications médicales sous forme de médicaments régénérateurs. L'invention concerne également des protocoles d'initiation et d'entretien, ainsi que d'expansion ex vivo de telles cellules souches provenant de sang cordonal. On montre en outre, qu'à partir de ces cellules, en employant des protocoles d'induction de différentiation variée, des progéniteurs de lignage distinct pour l'hématopoïese et l'endothèle, des progéniteurs mésenchymateux pour des muscles, os, cartilages et graisses, ainsi que des progéniteurs neuraux, peuvent être cultivés et amplifiés pour l'usage en médecine de régénération.

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