Human epithelial cell matrix and uses therefor

Details Human epithelial cell matrix and uses therefor Human epithelial cell matrix and uses therefor

C - Chemistry, Metallurgy

07

K

C07K 14/78 (2006.01) A61C 8/00 (2006.01) A61K 35/36 (2006.01) A61K 38/39 (2006.01) A61L 27/00 (2006.01) A61L 27/22 (2006.01) A61L 27/34 (2006.01) A61L 27/38 (2006.01) A61L 27/60 (2006.01) A61L 31/10 (2006.01) C12N 5/00 (2006.01) C12N 5/06 (2006.01) C12N 5/08 (2006.01)

Patent

CA 2252421

A method of growing epithelial cells by contacting the cells with the extracellular matrix deposited or secreted by human MCF-10A mammary epithelial cells. The MCF-10A matrix stimulates cell attachment and hemidesmosome formation in epithelial cells contacted therewith. The invention also encompasses the isolated or purified MCF-10A matrix, compositions comprising the matrix, and shaped articles coated with the matrix.

Cette invention se rapporte à un procédé permettant d'obtenir la croissance de cellules épithéliales en mettant en contact ces celllules avec la matrice extra-cellulaire déposée ou sécrétée par des cellules épithéliales mammaires MCF-10A. Cette matrice MCF-10A stimule la fixation des cellules et la formation d'hémidesmosomes dans les cellules épithéliales mises en contact avec ladite matrice. Cette invention décrit également la matrice MCF-10A isolée ou purifiée, des compositions comprenant cette matrice et des articles façonnés enrobés de cette matrice.

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