Human gamma, delta t cell antigen receptor polypetides and...

C - Chemistry – Metallurgy – 12 – N

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230/13, 167/37,

C12N 15/12 (2006.01) C07K 14/725 (2006.01) C07K 16/28 (2006.01) C12N 5/18 (2006.01) G01N 33/566 (2006.01)

Patent

CA 1340140

The present invention is directed to a form of the human gamma T cell antigen receptor polypeptide termed Form 2bc, which has a molecular weight of about 40,000 daltons, and comprises a constant region containing a sequence encoded by only two C.gamma.2 CII exon copies. The invention also relates to T cell antigen receptor heterodimers comprising the Form 2bc gamma polypeptide, and to nucleic acid sequences encoding the Form 2bc gamma polypeptide and portions thereof. Also provided is a method for producing expression of a gamma, delta T cell antigen receptor heterodimer. The invention also related to monoclonal antibodies specifically reactive with an epitope of the gamma or delta polypeptides. In specific embodiments, these antibodies are reactive with the delta constant region, the delta variable region, or gamma constant region. Such antibodies can be identified by detecting co-modulation of the CD3 antigen.

581613

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