Human growth hormone produced by recombinant dna in mouse cells

Details Human growth hormone produced by recombinant dna in mouse cells Human growth hormone produced by recombinant dna in mouse cells

C - Chemistry, Metallurgy

12

N

195/1.17, 195/1.

C12N 5/02 (2006.01) C07K 14/61 (2006.01) C12N 15/00 (2006.01) C12N 15/86 (2006.01)

Patent

CA 1224168

HUMAN GROWTH HORMONE PRODUCED BY RECOMBINANT DNA IN MOUSE CELLS Abstract of the Disclosure A recombinant DNA composed of (1) bovine papilloma virus, (2) the promoter region of the mouse metallothionein I gene and (3) human growth hormone structural sequences ligated to the metallothionein pro- moter was constructed. The recombinant was stably main- tained as an episome and directed production of human growth hormone when introduced into cultured mammalian cells. Not only was the yield unexpectedly high, puri- fication was vastly simplified because the growth hor- mone was secreted into the tissue culture medium. The process was suitable for spinner culture. Additionally, recombinant DNA molecules composed of (1) bovine papil- loma virus and (2) the whole metallothionein I gene were utilized to render mouse cells resistant to toxic con- centrations of cadmium. This combination was utilized as a selective marker to cotransfer other, non-select- able genes (such as human growth hormone) into mammalian cells.

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