Human hematopoietic stem and progenitor cell antigen and...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/12 (2006.01) C12N 5/0789 (2010.01) C07K 14/705 (2006.01) C07K 16/28 (2006.01) G01N 33/554 (2006.01) G01N 33/569 (2006.01)

Patent

CA 2251604

A hematopoietic progenitor cell antigen and reagents, notably antibodies, that specifically bind to the antigen are provided. Expression of the antigen is highly tissue specific. It is only detected on a subset of hematopoietic progenitor cells derived from human bone marrow, fetal bone marrow and liver, cord blood and adult peripheral blood. The subset of cells recognized by AC133 is CD34bright and contains substantially all of the CFU-GM activity present in the CD34+ population. This highly specific distribution of AC133 makes it exceptionally useful as a reagent for isolating and characterizing human hematopoietic progenitor and stem cells. Cells selected for expression of AC133 antigen can be further purified by selection for other hematopoietic stem cell and progenitor cell markers.

L'invention concerne un antigène de cellules hématopoïétiques parentes et des réactifs, en particulier des anticorps, se fixant spécifiquement à l'antigène. L'expression de l'antigène est strictement spécifique de certains tissus. Elle n'est détectée que dans un sous-ensemble de cellules hématopoïétiques parentes dérivées de la moelle épinière humaine, de la moelle épinière et du foie de foetus, du sang du cordon ombilical et du sang périphérique de l'adulte. Le sous-ensemble de cellules reconnues par AC133 est le sous-ensemble CD34?brillant¿ et il contient sensiblement toute l'activité CFU-GM présente dans la population CD34?+¿. Cette répartition, hautement spécifique de AC133, le rend exceptionnellement utile en tant que réactif pour isoler et caractériser des cellules hématopoïétiques souches ou parentes. Les cellules choisies pour l'expression de l'antigène AC133 peuvent, en outre, être purifiées par sélection d'autres marqueurs de cellules hématopoïétiques souches et parentes.

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