Human interferon-.beta.2a and interferon-.beta.2b; vectors...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/20 (2006.01) A61K 38/16 (2006.01) A61K 38/21 (2006.01) C07K 14/54 (2006.01) C07K 14/565 (2006.01) A61K 38/00 (2006.01)

Patent

CA 1341369

Human interferon-beta2A and interferon-beta2B are produced in purified form by recombinant DNA techniques. Two separate human genes have been identified which code for the production of IFN-.beta.2A and IFN- .beta.2B, respectively. The sequence of IFN-.beta.2A cDNA is established. These genes and cDNA have been cloned into mammlian cells with an SV40 early promoter sequence and such genomic clones are capable of pro- ducing IFN-.beta.2A and IFN-.beta.2B. The antaviral activity of such recombinant IFN-.beta.2A and IFN-.beta.2B is demonstrated as well as other biological activity identifying them as human interferons. It has been shown that IFN-.beta.2 secretion is induced in human cells by growth- stimulatory cytokines.

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