Human interleukin 6 inhibitor

C - Chemistry – Metallurgy – 07 – K

Patent

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Details

C07K 14/715 (2006.01) A61K 38/20 (2006.01) C07K 14/47 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2155629

A previously undescribed Interleukin-6 inhibitor activity has been successfully isolated from the supernatant of the human promyelocytic leukemia cell line HL-60. Treatment of the HL-60 cell line with cycloheximide prevents the appearance of the inhibitory activity in the cellular supernatant. Incubation of the HL-60 supernatant with trypsin destroys the activity. The above observations indicate the inhibitor is a protein. Membrane and gel filtration studies indicate the protein has a molecular weight between 10,000 and 30,000 daltons. The inhibitor was partially isolated from other proteins by dye-ligand and reverse phase chromatography.

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