Human leukocyte antigen typing

Details Human leukocyte antigen typing Human leukocyte antigen typing

C - Chemistry, Metallurgy

12

Q

150/3, 150/8.5

C12Q 1/68 (2006.01)

Patent

CA 2035813

ABSTRACT A method of human HLA-DR and/or Dw allotype matching is provided. The process comprises effecting polymerase chain reaction (PCR) amplification of a HLA-DRB gene exon 2 nucleotide sequence of a first sample of DNA. The DNA fragments resulting from this amplification are then separated according to size and the length polymorphism of these separated fragments is then determined. Thereafter, the determined length polymorphism is compared with the length polymorphism of DNA fragments resulting from PCR amplification of the HLA-DRB gene exon 2 nucleotide sequence of a second sample of DNA.

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