Human metabolic models and methods

G - Physics – 06 – F

Patent

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G06F 19/12 (2011.01) G01N 33/48 (2006.01)

Patent

CA 2480216

The invention provides in silico models for determining the physiological function of human cells, including human skeletal muscle cells. The models include a data structure relating a plurality of Homo sapiens reactions, a constraint set for the plurality of Homo sapiens reactions, and commands for determining a distribution of flux through the reactions that is predictive of a Homo sapiens physiological function. A model of the invention can further include a gene database containing information characterizing the associated gene or genes. A regulated Homo sapiens reaction can be represented in a model of the invention by including a variable constraint for the regulated reaction. The invention further provides methods for making an in silico Homo sapiens physiological function using a model of the invention.

L'invention porte sur des modèles in silico servant à déterminer les fonctions physiologiques de cellules humaines dont celles des muscles du squelette. Lesdits modèles comportent une structure de données associant différentes réactions de l'<i>Homo sapiens</i>, une contrainte imposée auxdites réactions, et des instructions déterminant la distribution de flux traversant les réactions, et projetant une fonction physiologique de l'<i>Homo sapiens</i>. Un tel modèle peut également comporter une base de données de gènes contenant des informations caractérisant le ou les gènes associés. On peut représenter une réaction régulée de l'<i>Homo sapiens</i> dans un modèle de l'invention en imposant une contrainte variable à la réaction régulée. L'invention porte également des méthodes d'élaborationin silico d'une fonction physiologique de l'<i> Homo sapiens</i> à l'aide d'un modèle de l'invention.

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