C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
195/1.102
C12N 15/00 (2006.01) C12N 5/02 (2006.01) C12P 21/00 (2006.01) G01N 33/577 (2006.01)
Patent
CA 1212913
ABSTRACT OF THE DISCLOSURE Human monoclonal antibody reagents for specific therapeutic and diagnostic use against such diseases as hepatitis, rabies and tetanus can be prepared from human peripheral blood lymphocytes especially selected for their capability to bind to a selected antigen. The selected lymphocytes are infected with Epstein Barr Virus and after culture are cloned by limiting dilution on irradiated feeder layers of autologous human mononuclear cells in the presence of T cell growth factor. Clones producing the antibody to the selected antigen are recovered and grown in bulk culture and the desired reagent prepared therefrom. Clones thus produced may be fused with thioguanine-resistant ouabain-resistant human myeloma cell lines so as to produce a hybridoma which is more stable, which has a higher cloning efficiency, and which produces more antibody.
406033
Gastle And Associates
Queen's University At Kingston
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