Human monoclonal antibodies against bacterial toxins

C - Chemistry – Metallurgy – 12 – N

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C12N 15/00 (2006.01) C07K 16/12 (2006.01) C12N 5/00 (2006.01) C12P 21/00 (2006.01) A61K 38/00 (2006.01)

Patent

CA 1262238

ABSTRACT The production of stable hybrid cell lines that secrete human monoclonal antibodies against bacterial toxins by fusing post-immunization human peripheral blood lymphocytes with nonsecretor mouse myeloma cells is described. Using the method, protective monoclonal antibodies against tetanus toxin and diphtheria toxin were produced that bind tetanus toxin and diphtheria toxin in vitro, respectively, and prevent tetanus and diphtheria in vivo in animals, respectively.

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