Human myoblast cell lines and their uses

Details Human myoblast cell lines and their uses Human myoblast cell lines and their uses

C - Chemistry, Metallurgy

12

N

C12N 5/077 (2010.01) A61K 35/34 (2006.01) A61K 38/00 (2006.01) A61K 39/00 (2006.01) A61K 48/00 (2006.01) A61P 21/04 (2006.01) C12N 5/10 (2006.01) C12N 15/85 (2006.01) C12N 15/87 (2006.01) C12Q 1/00 (2006.01) G01N 33/15 (2006.01) G01N 33/50 (2006.01)

Patent

CA 2307104

Described is a human muscular cell line which is able to proliferate and generated from primary human muscular cells by a process comprising the step of: a) pre-treating a culture of said primary human muscular cells or a suspension thereof with at least one glucocorticoid, b) optional step comprising obtaining a suspension of said pre-treated culture of step a), c) transferring into the pre-treated cells of the suspension of step a) or b) at least one nucleic acid vector which is not of retroviral origin and which is competent to immortalize said pre-treated cells and d) culturing the transferred cells of step c). Furthermore, human muscular cells derived from said cell lines are provided as well as pharmaceutical and diagnostic compositions comprising the same.

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