Human nerve growth factor

Details Human nerve growth factor Human nerve growth factor

C - Chemistry, Metallurgy

07

K

530/13, 530/3.18

C07K 14/48 (2006.01) C07K 1/36 (2006.01) A61K 38/00 (2006.01)

Patent

CA 1339791

A process is disclosed for the preparation of a biologically active subunit ( .beta. subunit) of human nerve growth factor (NGF) from human placenta. The process involves homogenizing the tissue of human placenta, dialysing the homogenized tissue at pH 4.0 in order to dissociate the 7S NGF form, and chromatographically fractionating the resultant solution on a three step cation exchange chromatography column. The active fraction can be purified by cation exchange chromatography or affinity chromatography on polyclonal antibodies. Pharmaceutical compositions containing the active subunit are also disclosed.

593792

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