Human neuronal nicotinic acetylcholine receptor compositions...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/12 (2006.01) C07H 21/04 (2006.01) C07K 14/705 (2006.01) C12Q 1/02 (2006.01) C12Q 1/68 (2006.01) G01N 33/566 (2006.01)

Patent

CA 2221311

Nucleic acid molecules encoding human neuronal nicotinic acetylcholine receptor alpha and beta subunits, mammalian and amphibian cells containing the nucleic acid molecules, and methods for producing alpha and beta subunits are provided. In particular, nucleic acid molecules encoding .alpha.6 subunits and molecules encoding .beta.3 subunits of human neuronal nicotinic acetylcholine receptors are provided. In addition, combinations of a plurality of subunits, such as one or more of .alpha.1, .alpha.2, .alpha.3, .alpha.4, .alpha.5, .alpha.6 and/or .alpha.7 subunits in combination with one or more of .beta.3 subunits or such as one or more of .beta.2, .beta.3 and/or .beta.4 subunits in combination with an .alpha.6 subunit are provided.

On décrit des molécules d'acides nucléiques codant des sous-unités alpha et béta du récepteur neuronal humain de l'acétylcholine nicotinique, des cellules de mammifères et d'amphibiens contenant ces molécules d'acide nucléique, et des procédés de production de ces sous-unités alpha et béta. On décrit particulièrement des molécules d'acides nucléiques codant des sous-unités .alpha.¿6? et des molécules codant des sous-unités .beta.¿3? des récepteurs neuronaux humains de l'acétylcholine nicotinique. On décrit en outre des combinaisons de plusieurs sous unités, telles qu'une sous-unité ou plus parmi .alpha.¿1?, .alpha.¿2?, .alpha.¿3?, .alpha.¿4?, .alpha.¿5?, .alpha.¿6? et/ou .alpha.¿7? combinées avec une ou plusieurs sous-unités .beta.¿3? ou telles qu'une ou plusieurs sous-unités parmi .beta.¿3?, .beta.¿3? et/ou .beta.¿4? combinées avec une sous-unité .alpha.¿6?.

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