Human pancreatic beta cell lines for diagnostic of diabetes

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 5/09 (2010.01) C12N 5/071 (2010.01) A61K 35/39 (2006.01) C12N 5/10 (2006.01) C12N 15/09 (2006.01) C12N 15/867 (2006.01) G01N 33/543 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2678870

The present invention relates to a method for preparing commercial scale quantities of human functional Betacells and to the establishmentofcell lines. It also relates to a method of diagnosis using Beta cell tumorsor cells derived thereof. The method comprisessub-transplantation procedure to enrich the graft in proliferating Betacells, allowingtogenerate human Betacell lines. Such lines express little amount of insulin and have a gene expression profile that resembles to adult Betacells. In addition, the human Betacell lines are able to normalize glycemia of diabetic mice when transplanted,demonstrating their insulin secretion capabilities.

La présente invention concerne un procédé de préparation de quantités à échelle commerciale de cellules bêta fonctionnelles humaines et d'établissement de lignées de cellules. L'invention concerne aussi une méthode de diagnostic utilisant des cellules tumorales bêta ou des cellules dérivées de celles-ci. Cette méthode comprend une opération de sous transplantation destinée à enrichir la greffe dans des cellules bêta proliférantes, permettant de générer des lignées de cellules bêta humaines. Ces lignées expriment une petite quantité d'insuline et possède un profil d'expression génique qui ressemble à celui de cellules bêta adultes. Par ailleurs, ces lignées de cellules bêta humaines sont capables de normaliser la glycémie de souris diabétiques lorsqu'elles sont transplantées, démontrant leur capacité de sécrétion d'insuline.

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