Human papilloma virus detection with dna microarray

Details Human papilloma virus detection with dna microarray Human papilloma virus detection with dna microarray

C - Chemistry, Metallurgy

12

Q

C12Q 1/70 (2006.01) G01N 33/569 (2006.01)

Patent

CA 2484681

A method is provided of detecting the presence of HPV comprising the following steps: a. amplification and labelling part of the E1 HPV gene, in particular its 3' end; b. hydrizing the labelled fragment to a solid support containing microarrays with various HPV specific capture probes; c. removing uncaptured labeled fragments; d. detecting captured detectable moiety indicating the presence of HPV sequence DNA in a sample. Further provided is a test kit for carrying out said detection method.

L'invention concerne un procédé permettant de détecter la présence d'un papillomavirus (HPV). Ce procédé comprend les étapes consistant à : a. amplifier et marquer une partie du gène E1 HPV, en particulier son extrémité 3' ; b. hybrider le fragment marqué à un support solide contenant des puces et auquel sont fixées des sondes de capture spécifiques de différents HPV ; c. retirer les fragments marqués non capturés ; d. détecter une fraction détectable capturée qui indique la présence de l'ADN d'une séquence de HPV dans un échantillon. L'invention concerne en outre un nécessaire d'analyse permettant d'effectuer ledit procédé de détection.

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