Human papilloma virus inhibition by a hairpin ribozyme

Details Human papilloma virus inhibition by a hairpin ribozyme Human papilloma virus inhibition by a hairpin ribozyme

C - Chemistry, Metallurgy

12

N

C12N 9/22 (2006.01) A61K 31/70 (2006.01) A61P 35/00 (2006.01) C07H 21/02 (2006.01) C12N 15/11 (2006.01) C12Q 1/34 (2006.01) C12Q 1/68 (2006.01) C12Q 1/70 (2006.01) A61K 48/00 (2006.01)

Patent

CA 2190102

Synthetic ribozymes, i.e. ribozymes including a hairpin portion, binding sites for binding to a human papilloma virus (HPV) after virsl base 419 or 434, respectively, and cleavage sites for cleaving HPV virus are disclosed. The invention also includes a diagnostic assay for determining the presence of HPV-16 in tissues based on PCR amplification.

On décrit des ribozymes synthétiques, c'est-à-dire de ribozymes comprenant une partie en forme d'épingle à cheveux, des sites de liaison sur un papillomavirus humain (HPV) après la base virale 419 ou 434, respectivement, et des sites de clivage du virus HPV. L'invention se rapporte également à des essais diagnostiques servant à déterminer la présence du HPV-16 dans des tissus par amplification PCR.

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