Human pluripotent hematopoietic colony stimulating factor,...

C - Chemistry – Metallurgy – 07 – K

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195/46, 195/78,

C07K 14/53 (2006.01) A61K 38/19 (2006.01) C12P 21/02 (2006.01) C12N 5/08 (2006.01)

Patent

CA 1340266

Highly purified pluripotent hematopoietic colony-stimulating factor (pluripotent CSF), a glycoprotein (MW 19,600) constitutively produced by human tumor cells has been highly purified from low serum-containing conditioned medium to apparent homogeneity. Pluripotent CSF supports the growth of human mixed colonies (CFU-GEMM), granulocyte-macrophage colonies (CFU-GM), early erythroid colonies (BFU-E) and induces differentiation of human leukemic cells. The specific activity of the purified pluripotent CSF in the CFU-GM assay is 1.5 X 10 8 U/mg protein.

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