Human protein c expression vector

Details Human protein c expression vector Human protein c expression vector

C - Chemistry, Metallurgy

12

N

C12N 15/57 (2006.01) C12N 5/10 (2006.01) C12N 9/64 (2006.01) C12N 15/67 (2006.01)

Patent

CA 2078667

2078667 9213079 PCTABS00014 An object of the present invention is to provide DNA fragments encoding human protein C and human protein C expression vectors including the same. The object is attained by removing arbitrary n (n = 1 to 6) introns from 7 introns distributing between 8 exons in human protein C gene, ligating the remaining before and behind sequences to form novel DNA fragments encoding human protein C and preparing expression vectors including the same and mammalian cells transformed with said vectors. Another object of the present invention is to provide DNA fragments of increased expression efficiency by incorporating a useful DNA fragment into the introns remaining in the DNA fragments. They are useful for production of human protein C.

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