Human tpa production using vectors coding for dhfr protein

C - Chemistry – Metallurgy – 12 – N

Patent

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195/1.22, 195/1.

C12N 15/00 (2006.01) C12N 9/06 (2006.01) C12N 9/72 (2006.01) C12N 15/85 (2006.01)

Patent

CA 1271149

ABSTRACT A method for producing tissue plasminogen activator (t-PA) in eukaryotic host cells is disclosed. Enhanced levels of t-PA production are obtained by co-amplification of the t-PA gene through treatment of cultures transformed with mutant or wild type DHFR with methotrexate.

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