Humanized green fluorescent protein genes and methods

Details Humanized green fluorescent protein genes and methods Humanized green fluorescent protein genes and methods

C - Chemistry, Metallurgy

12

N

C12N 15/12 (2006.01) A61K 49/00 (2006.01) C07K 14/435 (2006.01) C07K 19/00 (2006.01) C12N 5/10 (2006.01) C12N 15/62 (2006.01) C12N 15/65 (2006.01) C12N 15/85 (2006.01) C12N 15/87 (2006.01) C12Q 1/02 (2006.01) C12Q 1/68 (2006.01) G01N 33/483 (2006.01)

Patent

CA 2243088

Disclosed are synthetic and "humanized" versions of green fluorescent protein (GFP) genes adapted for high level expression in mammalian cells, especially those of human origin. Base substitutions are made in various codons in order to change the codon usage to one more appropriate for expression in mammalian cells. Recombinant vectors carrying such humanized genes are also disclosed. In addition, various methods for using the efficient expression of humanized GFP in mammalian cells and in animals are described.

L'invention se rapporte à des versions synthétiques et "humanisées" des gènes de la protéine vert fluorescent adaptés pour un haut niveau d'expression dans les cellules mammaliennes, notamment celles d'origine humaine. Des substitutions de base sont effectuées dans divers codons afin de modifier l'usage du codon en un usage plus approprié à une expression dans les cellules mammaliennes. Des vecteurs de recombinaisons portant ces gènes humanisés sont également décrits, ainsi que divers procédés d'utilisation de l'expression efficace de la protéine vert fluorescent humanisée dans les cellules mammaliennes et chez les animaux.

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