Hybrid cell line for producing complement-fixing monoclonal...

C - Chemistry – Metallurgy – 12 – N

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195/1.11

C12N 5/02 (2006.01) A61K 39/395 (2006.01) C12N 15/00 (2006.01) C12P 21/00 (2006.01)

Patent

CA 1213841

ABSTRACT OF THE DISCLOSURE Hybrid cell line for production of monoclonal antibody to mouse I-A antigen and to I-A analogous antigen on human and other animal lymphocytes is described. The hybrid is formed by fusing P3X63-Ag8-653 myeloma cells with spleen cells from A.TH (H-2t2) mouse immunized with A.TL (H-2t1) mouse lymphocytes. The monoclonal antibody reacts with I-A analo- gous antigens in a wide range of animal species.

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