Hybrid dna synthesis of insulin

C - Chemistry – Metallurgy – 12 – N

Patent

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195/1.26, 195/1.

C12N 15/17 (2006.01) C07K 14/395 (2006.01) C07K 14/62 (2006.01) C12N 15/62 (2006.01) C12N 15/81 (2006.01)

Patent

CA 1341372

Methods and compositions are provided for efficient production of polypeptides having insulin activity. The proinsulin gene is joined to secretion and processing signals far expression and secretion in yeast. The product may be obtained in enhanced yield in the nutrient medium. Cell line pYinsl was deposited at the A.T.C.C. on April 6, 1983 and given Accession No. 20670.

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