Hybrid phthalocyanine derivatives and their uses

C - Chemistry – Metallurgy – 09 – B

Patent

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Details

C09B 47/04 (2006.01) C07D 487/22 (2006.01) C07F 7/08 (2006.01) C07F 7/10 (2006.01) G01N 33/533 (2006.01) G01N 33/76 (2006.01)

Patent

CA 2215727

Water soluble hybrid phthalocyanine derivatives useful in competitive and noncompetitive assays immunoassays, nucleic acid and assays are disclosed and claimed having (1) at least one donor subunit with a desired excitation peak; and (2) at least one acceptor subunit with a desired emission peak, wherein said derivative(s) is/are capable of intramolecular energy transfer from said donor subunit to said acceptor subunit. Such derivatives also may contain an electron transfer subunit. Axial ligands may be covalently bound to the metals contained in the water soluble hybrid phthalocyanine derivatives. Ligands, ligand analogues, polypeptides, proteins and nucleic acids can be linked to the axial ligands of the dyes to form dye conjugates useful in immunoassays and nucleic acid assays.

Cette invention concerne des dérivés hybrides de phthalocyanine solubles dans l'eau et utiles dans des dosages, des dosages immunologiques et des dosages d'acides nucléiques compétitifs ou non, lesquels dérivés comportent (1) au moins une sous-unité donatrice ayant un pic d'excitation voulu, et (2) au moins une sous-unité d'acceptation ayant un pic d'émission voulu. Ce ou ces dérivés sont capables d'effectuer un transfert d'énergie intramoléculaire depuis ladite sous-unité donatrice vers ladite sous-unité d'acceptation. Ces dérivés peuvent également contenir une sous-unité de transfert d'électrons. Des ligands axiaux peuvent être liés par covalence aux métaux contenus dans les dérivés hybrides de phthalocyanine solubles dans l'eau. Des ligands, des analogues de ligands, des polypeptides, des protéines et des acides nucléiques peuvent être liés aux ligands axiaux de colorants de sorte que des conjugués de colorants utiles dans des dosages immunologiques et des dosages d'acides nucléiques soient formés.

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