Hybrid plasmid and process for producing glutathione using...

Details Hybrid plasmid and process for producing glutathione using... Hybrid plasmid and process for producing glutathione using...

C - Chemistry, Metallurgy

12

N

195/1.22, 195/12

C12N 1/20 (2006.01) C07K 5/02 (2006.01) C12N 9/00 (2006.01) C12N 15/00 (2006.01) C12P 21/02 (2006.01)

Patent

CA 1220734

ABSTRACT OF THE DISCLOSURE A hybrid plasmid that can be incorporated into a bacterium of the species Escherichia coli for use in the production of glutathione is disclosed. This hybrid plasmid has incorporated into an E. coli vector the genes for .gamma.-glutamyl-L-cysteine synthetase and/or glutathione synthetase, which are the two enzymes involved in glutathione synthesis. Also disclosed is a process for producing glutathione which comprises culturing a bacterium of E. coli having said hybrid plasmid, and recovering glutathione from the culture broth or producing glutathione by bringing L-glutamic acid, L-cysteine, glycine, adenosine-5'-triphosphate and magnesium ions into contact with a material obtained by treating the microbial cells of E. coli.

438038

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