Hybrid plasmid with marker

C - Chemistry – Metallurgy – 12 – N

Patent

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195/1.18

C12N 15/00 (2006.01) C12N 9/06 (2006.01) C12N 15/81 (2006.01) C12N 15/82 (2006.01)

Patent

CA 1214409

Abstract of the Invention Recombinant plasmids adopted for transformation of prokaryotic and eukaryotic hosts having an insertion site for at least one DNA fragment are provided. In a preferred embodiment, nucleotide sequences coding for methotrexate-resistant dehydrofolate reductase and a nucleotide sequence, a portion thereof coding for a promotor, are ligated into the insertion site.

446364

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