Hybrid plasmids having a streptomycetes replicon and an...

C - Chemistry – Metallurgy – 12 – N

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195/1.21, 195/1.

C12N 15/00 (2006.01) C12N 15/76 (2006.01)

Patent

CA 1246474

Abstract of the disclosure: Starting from the Streptomycetes plasmids pSG2, pSG5 and pSVH1, "shuttle vectors" which, in addition to a E. coli replicon, also contain suitable selection mar- kers for E. coli and Streptomycetes are constructed. These shuttle vectors replicate both in E. coli and in Streptomycetes, and they can be used for cloning and expression of foreign DNA.

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