Hybrid portable origin of replication plasmids

Details Hybrid portable origin of replication plasmids Hybrid portable origin of replication plasmids

C - Chemistry, Metallurgy

12

N

C12N 15/69 (2006.01)

Patent

CA 2622710

The invention relates to modifying plasmid origins of replication to create hybrid origins of replication containing nucleotide sequences from more than one plasmid. The invention also relates to a modified origin of replication cassette that is portable or exchangeable due to the creation of multiple cloning sites flanking the origin of replication. Methods and plasmids for use in exchanging origins of replication are disclosed. Such modified or hybrid plasmids provide useful cloning tools that allow for regulation of the level of expression of a desired protein.

L'invention se rapporte à la modification des origines de plasmides pour créer des origines de réplication hybrides contenant des séquences nucléotidiques provenant de plus d'un plasmide. L'invention se rapporte également à une origine modifiée d'une cassette de réplication qui est rendue transférable ou échangeable par la création de sites de clonage multiples de part et d'autre de l'origine de réplication. L'invention concerne en outre des procédés et des plasmides permettant d'échanger les origines de réplication Ces plasmides modifiés ou hybrides constituent des outils de clonage qui peuvent servir à réguler le niveau d'expression d'une protéine particulière.

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