C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/62 (2006.01) C07K 14/46 (2006.01) C12N 1/21 (2006.01) C12N 9/00 (2006.01) C12N 9/16 (2006.01) C12N 9/86 (2006.01) C12Q 1/68 (2006.01) G01N 33/535 (2006.01) G01N 33/573 (2006.01)
Patent
CA 2019665
ABSTRACT OF THE DISCLOSURE The present invention provides hybrid poroteins between a fragment of an extracytoplasmic protein Pl, a protein P2 and an extracytoplasmic enzyme, the protein P2 being, in particular, an antigen or a single-chain variable frag- ment (Fv) of an antiboby, to a process for preparing them and also to their application, in particular, as a diagnostic agent and in the screening of a nucleic acid library or in the selection of recombinant clones. The nucleic acid sequence, consists of a hybrid sequence comprising successively the leader sequence of the structural gene for a suitable protein ?1 exported or secreted by a microorganism, in particular chosen from the group which comprises bacteria and yeasts, a nucleic acid sequence coding for a NH2 terminal fragment or the mature protein P1, a nucleic acid sequence coding for a protein P2 and then a fragment coding for at least one functional fragment of the mature sequence of a suitable extracyto- plasmic enzyme, the assembly of these fragments being in a single reading frame and coding for a hybrid protein possessing simultaneously the properties of the enzyme and some properties of the protein P2, in particular that of interacting specifically with an antibody, an antigen or a receptor.
Boquet Paul
Boulain Jean-Claude
Ducancel Frederic
Gillet Daniel
Menez Andre
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