Hybrid proteins produced by an ultrahigh prokaryotic...

Details Hybrid proteins produced by an ultrahigh prokaryotic... Hybrid proteins produced by an ultrahigh prokaryotic...

C - Chemistry, Metallurgy

12

N

530/13, 195/1.22

C12N 15/00 (2006.01) C07K 13/00 (1985.01)

Patent

CA 1265077

R126 ABSTRACT OF THE DISCLOSURE Hybrid useful proteins are prepared by a novel biological system comprising a prokaryotic host trans- formed with novel hybrid plasmids' .beta.-glucuronidase (BG) gene DNA and the desired protein gene DNA. Specifically exemplified are plasmids which comprise BG gene DNA and protein A DNA. E. coli K-12 derivative hosts transformed with plasmid pBG3-2.DELTA.N express >60% of the desired fusion protein having protein A-like biological activity. Other useful proteins can be expressed via the elegant highly efficient expression system of the subject inven- tion.

502064

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