C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01)
Patent
CA 2286287
A method is disclosed for detecting single-stranded target nucleic acid (2) which comprises the steps of forming a hybrid between said target nucleic acid and a nucleic acid probe (4), said nucleic acid probe labelled with an enzyme reagent (6) which hydrolyses single-stranded nucleic acid but is substantially without effect on double-stranded nucleic acid, said hybrid formed under conditions of pH which are outside the activity range of said enzyme reagent, adjusting said pH to a value within the activity range of said enzyme reagent, allowing said enzyme reagent substantially to hydrolyse any single-stranded nucleic acid present, and detecting said hybrid.
L'invention concerne un procédé de détection d'un acide nucléique cible monocaténaire (2), comprenant les étapes consistant à former un hybride entre cet acide nucléique cible et une sonde d'acide nucléique (4) marquée à l'aide d'un réactif enzymatique (6) qui hydrolyse l'acide nucléique monocaténaire mais est sensiblement dépourvu d'effet sur l'acide nucléique bicaténaire, l'hybride se formant dans des conditions de pH se situant en dehors de la plage d'activité du réactif enzymatique, puis à régler le pH afin que la valeur de celui-ci se situe dans la plage d'activité du réactif enzymatique, à permettre à ce réactif de sensiblement hydrolyser tout acide nucléique monocaténaire présent, et enfin à détecter cet hybride.
Harbron Stuart
Mbm & Co.
Zetatronics Limited
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