Hybridization assay for campylobacter rrna

Details Hybridization assay for campylobacter rrna Hybridization assay for campylobacter rrna

C - Chemistry, Metallurgy

07

H

167/44, 195/1.12

C07H 21/02 (2006.01) C07H 21/00 (2006.01) C12Q 1/68 (2006.01) G01N 33/53 (2006.01) G01N 33/543 (2006.01) G01N 33/569 (2006.01)

Patent

CA 2028012

ABSTRACT Method of detecting the presence of one or more Campylobacter species in a test sample, and providing a plurality of oligonucleotide probes, each having a nucleotide sequence which is complementary to regions of Campylobacter 16S rRNA. The assay format consists of a solution hybridization of at least two labelled oligonucleotide probes one of which is specific to a region of a Campylobacter rRNA, and then the capture of the resulting hybrid complex on a solid support for subsequent detection and quantitation. The assay has a quantitative sensitivity to detect 1.0 x 104 bacterium per ml of test sample. A test kit and apparatus suitable for the method are described. In an expanded variation of the method an assay and kit are described for detecting the presence of one or more target nucleic acid sequences in a test sample.

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