C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01)
Patent
CA 2434854
This invention demonstrates that lower molecular weight dextran sulfate is an effective volume exclusion agent of hybridization reactions. The hybridization buffers of this invention utilize smaller polymers of dextran sulfate, and thus are of a lower viscosity than conventional hybridization buffers that use higher molecular weight dextran sulfate polymers as volume exclusion agents. The lower viscosity of these low molecular weight dextran sulfate polymer buffers makes them useful for automated hybridization processes as in dispensing, automated flow, and mixing on slide.
L'invention concerne du dextran-sulfate à faible poids moléculaire qui est un agent d'exclusion de volume efficace destiné à des réactions d'hybridation. Les tampons d'hybridation de cette invention utilisent des polymères plus petits de dextran-sulfate, et présentent donc une viscosité moins importante que celle des tampons d'hybridation traditionnels qui utilisent des polymères de dextran-sulfate à poids moléculaire plus élevé comme agents d'exclusion de volume. La viscosité inférieure de ces tampons polymères de dextran-sulfate à faible poids moléculaire les rend utiles dans des processus d'hybridation automatisés, tels que la dispensation, le flux automatisé et le mélange sur micro-plaquette.
Christensen Kimberly
Utermohlen Joseph
Wolf Catherine
Mbm Intellectual Property Law Llp
Ventana Medical Systems Inc.
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