Hybridization compositions and methods

Details Hybridization compositions and methods Hybridization compositions and methods

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2724638

The invention provides methods and compositions for hybridizing at least one molecule to a target. The invention may, for example, eliminate the use of, or reduce the dependence on formamide in hybridization. Compositions for use in the invention include an aqueous composition comprising at least one nucleic acid sequence and at least one polar aprotic solvent in an amount effective to denature double-stranded nucleotide sequences.

L'invention porte sur des procédés et des compositions pour hybrider au moins une molécule à une cible. L'invention peut par exemple éliminer l'utilisation du formamide ou réduire la dépendance vis-à-vis de celui-ci au cours de l'hybridation. Les compositions de l'invention destinées à être utilisées comprennent une composition aqueuse comprenant au moins une séquence d'acide nucléique et au moins un solvant aprotique polaire dans une quantité efficace pour dénaturer des séquences nucléotidiques double brin.

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