Hybridization device and method

Details Hybridization device and method Hybridization device and method

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) B01L 3/00 (2006.01) C12M 1/34 (2006.01) G01N 35/00 (2006.01)

Patent

CA 2474020

An apparatus and method for DNA hybridization is provided. The apparatus and method work in conjunction with a substrate comprising an upper surface having probes. The apparatus may comprise a material which abuts the substrate, with at least a portion of the material being pliable. The material and the substrate form a plurality of chambers, each chamber having a bottom including at least a portion of the upper surface, at least one sidewall, and an opening. The apparatus further comprises a mechanism for closing the openings of the chambers, thereby sealing the chambers.

L'invention concerne un appareil et un procédé d'hybridation d'ADN, qui opèrent en combinaison avec un substrat (20) comprenant une surface supérieure équipée de sondes (22). L'appareil peut comprendre un matériau (62) jouxtant le substrat (20), une partie dudit matériau (62) étant souple. Le matériau (62) et le substrat (20) forment plusieurs chambres (70), chacune (70) ayant un fond comportant au moins une partie de la surface supérieure (20), au moins une paroi latérale (64) et une ouverture (68). L'appareil comprend en outre un dispositif conçu pour fermer les ouvertures (68) des chambres (70), fermant ainsi hermétiquement lesdites chambres (70).

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