Hybridization linkers

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 9/24 (2006.01) C07H 21/00 (2006.01) C07K 1/00 (2006.01) C07K 19/00 (2006.01)

Patent

CA 2750874

The invention provides method of covalently coupling two or more moieties, the method comprising: (a) providing a first moiety having covalently attached thereto (i) at least one first linker comprising a first hybridizable region and (ii) at least one first group capable of forming a covalent bond; (b) providing a second moiety having covalently attached thereto (i) at least one second linker comprising a second hybridizable region capable of hybridizing to the first hybridizable region and (ii) at least a second group capable of forming a covalent bond with the first group; (c) contacting the first and second moieties under conditions that allow the first and second hybridizable regions to hybridize and link the moieties; and (d) exposing the linked moieties to conditions that allow the formation of a covalent bond between the first and second groups.

La présente invention concerne un procédé permettant de lier de manière covalente deux fragments ou plus, le procédé consistant à : (a) fournir un premier fragment auquel sont fixés de manière covalente (i) au moins un premier lieur comprenant une première région hybridable et (ii) au moins un premier groupe pouvant former une liaison covalente; (b) fournir un second fragment auquel sont fixés de manière covalente (i) au moins un second lieur comprenant une seconde région hybridable pouvant s'hybrider avec la première région hybridable et (ii) au moins un second groupe pouvant former une liaison covalente avec le premier groupe; (c) mettre le premier fragment en contact avec le second fragment dans des conditions qui permettent que les première et seconde régions hybridables s'hybrident et lient les fragments; et (d) exposer les fragments liés à des conditions qui permettent la formation d'une liaison covalente entre le premier et le second groupe.

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