Hybridization portion control oligonucleotide and its uses

Details Hybridization portion control oligonucleotide and its uses Hybridization portion control oligonucleotide and its uses

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2469383

The present invention relates to an oligonucleotide for analyzing a target nucleotide sequence by hybridization and its applications. The oligonucleotide has the following general structure. 5'-Xp-Yq-Zr-3' or 5'-Zr-Yq-Xp-3' Wherein Xp represents a first hybridization portion having a specific hybridizing nucleotide sequence substantially complementary to said target nucleotide sequence in said sample nucleic acid to hybridize therewith; Yq represents a regulator portion comprising at least two universal bases or non- discriminatory base analogs; Zr represents a second hybridization portion having a pre-selected arbitrary nucleotide sequence; p, q and r represent the number of nucleotides; and X,Y and Z is deoxyribonucleotide or ribonucleotide.

La présente invention concerne un oligonucléotide permettant d'analyser une séquence nucléotidique cible par hybridation, et ses applications. Cet oligonucléotide possède la structure générale suivante: 5'-X¿p?-Y¿q?-Z¿r?-3' ou 5'-Z¿r?-Y¿q?-X¿p?-3'. Dans cette structure, X¿p? représente une première partie d'hybridation possédant une séquence nucléotidique d'hybridation spécifique sensiblement complémentaire de la séquence nucléotidique cible de l'acide nucléique de l'échantillon à hybrider avec la première, Y¿q? représente une partie de régulateur comprenant au moins deux bases universelles ou deux analogues de base non discriminatoires, Z¿r? représente une seconde partie d'hybridation possédant une séquence nucléotidique arbitraire présélectionnée, p, q et r représentent le nombre de nucléotides et X, Y et Z sont désoxyribonucléotidiques ou ribonucléotidiques.

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