Hybridization probe assay and array

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01)

Patent

CA 2636872

A probe suitable for coupling with a particulate support such as a microbead, the probe comprising: a) a coupling group which permits coupling of the probe to the surface of the particulate support; b) a spacer; and c) a target- specific oligonucleotide probe sequence, wherein the spacer comprises: i) an oligonucleotide spacer of at least 15 nucleotides between the target specific probe sequence and the support coupling group; and optionally ii) a carbon spacer of between 3 and 50 carbon units between the target specific probe sequence and the support coupling group.

La présente invention concerne une sonde adéquate pour le couplage d~un support particulaire tel qu~une microbille, la sonde comprenant : a) un groupe de couplage qui permet le couplage de la sonde à la surface du support particulaire, b) une pièce d~écartement et c) une séquence de sonde oligonucléotidique spécifique à la cible, sachant que la pièce d~écartement comprend : i) une pièce d~écartement oligonucléotidique d~au moins 15 nucléotides entre la séquence de sonde spécifique cible et le groupe de couplage de support et, en option ii) une pièce d~écartement en carbone de 3 à 50 unités de carbone entre la séquence de sonde spécifique à la cible et le groupe de couplage de support.

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