Hybridization probes which differentiate between the...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01)

Patent

CA 2317897

The invention provides a method to differentiate between bacteria from the Actinomadura madurae group of bacteria and the Maduromycetes taxa using two nucleic acid probes that are complementary to a conserved region of the Maduromycetes 16S rRNA genes and to a conserved region of the Actinomadura madurae group of bacteria 16S rRNA genes, respectively. The probes permit the rapid detection of DNA coding for the 16S rRNA in a sample differentiating the Actinomadura madurae group of bacteria from Maduromycetes within the Actinomycetales order as well as the A. madurae group of bacteria and Maduromycetes from streptomycetes. The method is accurate and reproducible.

L'invention concerne un procédé permettant de faire la differenciation entre des bactéries du groupe Actinomadura madurae et les bactéries Maduromicetes taxa au moyen de deux sondes d'acide nucléique qui sont complémentaires d'une région conservée des gènes codant pour l'ARN ribosomique 16S de Maduromycetes, et d'une région conservée des gènes codant pour l'ARN ribosomique du groupe de bactéries Actinomadura madurae. Lesdites sondes permettent une détection rapide d'ADN codant pour l'ARN ribosomique 16S dans un échantillon, la différenciation étant faite entre le groupe de bactéries Actinomadura madurae et les bactéries Maduromicetes de l'ordre des Actinomycetales, ainsi qu'entre d'une part, le groupe de bactéries A. madurae et les bactéries Maduromycetes et, d'autre part, les streptomycetes. Le procédé présenté est précis et reproductible.

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