C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01)
Patent
CA 2480164
The invention relates to kits and methods for hybridizing nucleic acids with a specific nucleic acid-binding agent, such as a complementary nucleic acid. Previously, others have hybridized a nucleic acid with such an agent bound to a substrate. The improved methods described herein comprise binding a polycationizable attractor compound to the substrate, in addition to the agent. Examples of suitable polycationizable attractor compounds include polypeptides, including those with tunable cationizable amino acid residues, such as histidine. Compositions, kits, devices, and methods that make use of this hybridization rate enhancement technology are disclosed.
L'invention concerne des kits et des proc~d~s permettant d'hybrider des acides nucl~iques avec un agent de liaison ~ un acide nucl~ique sp~cifique tel qu'un acide nucl~ique compl~mentaire. Auparavant, d'autres ont d~j~ hybrid~ un acide nucl~ique avec un tel agent li~ ~ un substrat. Les proc~d~s am~lior~s de cette invention consistent ~ lier un compos~ attracteur susceptible de se polycationiser au substrat, en plus de l'agent. Des exemples de compos~s attracteurs adapt~s susceptibles de se polycationiser comprennent des polypeptides, notamment ceux poss~dant des r~sidus d'acides amin~s susceptibles de se cationiser ajustables tels que l'histidine. L'invention concerne ~galement des compositions, des kits, des dispositifs et des proc~d~s mettant en oeuvre cette technologie d'augmentation du taux d'hybridation.
Gunning Kerry B.
Hogan Michael
Powdrill Tom
Sigma-Genosys Lp
Smart & Biggar
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