Hybridoma and production of biologically active substance

Details Hybridoma and production of biologically active substance Hybridoma and production of biologically active substance

C - Chemistry, Metallurgy

12

P

C12P 21/08 (2006.01) C07K 14/50 (2006.01) C12N 5/12 (2006.01) C12N 5/22 (2006.01)

Patent

CA 2043535

TITLE: HYBRIDOMA AND PRODUCTION OF BIOLOGICALLY ACTIVE SUBSTANCE ABSTRACT OF THE DISCLOSURE Disclosed are (1) a hybridoma carrying a vector for expressing a fibroblast growth factor (FGF) protein gene; (2) a method for producing the hybridoma of (1) which comprises transforming a hybridoma with the vector for expressing the FGF protein gene; and (3) a method for producing a biologically active substance which comprises cultivating in a culture medium the hybridoma obtained by the method of (2) using a hybridoma producing a biologically active substance other than the FGF protein, producing the FGF protein and producing and accumulating the biologically active substance in a culture, and recovering the biologically active substance, whereby the biologically active substance can be efficiently produced and recovered using the serum-free medium, which is advantageous for industrial production and very useful for an improvement in the breeding of the hybridoma.

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