Hybridoma ct43 producing a monoclonal antibody to a mucin...

C - Chemistry – Metallurgy – 12 – P

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C12P 21/08 (2006.01) C07K 14/47 (2006.01) C07K 16/18 (2006.01) C07K 16/30 (2006.01) C12N 5/20 (2006.01) G01N 33/574 (2006.01) G01N 33/577 (2006.01)

Patent

CA 2065874

The present invention relates to a novel monoclonal antibody reactive with human colorectal mucin antigen. More particu- larly, the antibody of the invention is a murine monoclonal antibody, CT43, reactive with a novel antigenic determinant on mucin antigen highly correlated with human colorectal cancer. The antigenic determinant found by the CT43 has been characterized as neuraminidase and proteinase K resistant, periodate sensitive and unreactive with the glycoconjugates of Table 2. Methods are provided for the detection and quantitation of the CT43 antigenic determinant and its correlation with colorectal cancer. CT43, and CT66 specific For the sialylated Lewis a and Lewis a antigen have been deposited with the American Type Culture Collection, as accession numbers ATCC HB 10217 and ATCC HB 10218, deposited September 6, 1989,

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